CRG/UPF Proteomics Unit

CORE FACILITIES

HEAD OF THE UNIT:
Eduard Sabidó

SCIENTISTS:
Eva Borràs, Melissa Bradbury, Cristina Chiva, Guadalupe Espadas, Daniel Mancera, Julia Morales, Roger Olivella Pujol, Mireia Ortega, Olga Pastor, Amanda Solé

Summary

The Proteomics Unit is a joint effort of the Universitat Pompeu Fabra and the Center of Genomic Regulation to create an innovative research infrastructure with a high-level of excellence and sustainability to support scientists in their research projects and thus, successfully advance forward the European science roadmap. The activities of the Proteomics Unit are focused onto the provision of high-quality proteomics services with strong added-value to the research community by i) offering state-of-the-art methods, advise and expertise to support basic and translational researchers; ii) developing new methods and techniques that keep the unit up to date and at the forefront of the proteomics field; and iii) training the community and actively disseminating proteomics science and methods.

The Proteomics Unit is part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (ProteoRed, Instituto Carlos III)” a national consortium of proteomics units that grants access to state-of-the-art proteomics technology to the national health system and the national biomedical research community. The Proteomics Unit has successfully organized in 2017 the “EMBO Practical Course on Targeted Proteomics: Experimental Design and Data Analysis”, the “Courses@CRG: Advanced proteomics course”, and the Annual Proteomics Symposium 2017.

Research Projects

The Proteomics Unit promotes technology-driven research as an essential task to develop new applications and thus improve the services offered to the users.

Main research highlights:

  • Study labile protein post-translational modifications.
  • Characterization of protein interactions in cancer and atherosclerosis.
  • Biomarker discovery and validation in cancer and cardiac pathologies.
  • Development of targeted proteomics methods.
  • Quality assessment of mass spectrometry-based proteomics experiments.

Publications

Chiva C, Olivella R, Borràs E, Espadas G, Pastor O, Solé A, Sabidó E.
“QCloud: A cloud-based quality control system for mass spectrometry-based proteomics laboratories.”
PLoS One, 13(1):e0189209. doi: 10.1371/journal.pone.0189209. eCollection 2018.

Borràs E, Sabidó E.
“What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry.”
Proteomics, 17(17-18). doi: 10.1002/pmic.201700180 (2017).

Espadas G, Borràs E, Chiva C, Sabidó E.
“Evaluation of different peptide fragmentation types and mass analysers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer.”
Proteomics, 17(9). doi: 10.1002/pmic.201600416 (2017).

Chiva C, Sabidó E.
“Peptide Selection for Targeted Protein Quantitation.”
J Proteome Res, 16(3):1376-1380. doi: 10.1021/acs.jproteome.6b00115. Epub 2017 Jan 30.

Sequeiros T, Rigau M, Chiva C, Montes M, Garcia-Grau I, Garcia M, Diaz S, Celma A, Bijnsdorp I, Campos A, Di Mauro P, Borrós S, Reventós J, Doll A, Paciucci R, Pegtel M, de Torres I, Sabidó E, Morote J, Olivan M.
“Targeted proteomics in urinary extracellular vesicles identifies biomarkers for diagnosis and prognosis of prostate cancer.”
Oncotarget, 8(3):4960-4976. doi: 10.18632/oncotarget.13634. (2017).