Biomolecular Screening and Protein Technologies Unit

CORE FACILITIES

HEAD OF THE UNIT:
Carlo Carolis

LABORATORY TECHNICIANS:
Miriam Alloza, Katrina Broadbent, Natalia Rodrigo, Anna Puig

Summary

The Biomolecular Screening & Protein Technologies Unit (BMS&PT) provides researchers at CRG/PRBB, external institutions and private companies with a state-of-the-art technology platform and broad knowledge and expertise to perform the following services:

  1. Full-service production of plasmid clones, mutants, fusion constructs, libraries using different recombinant cloning technologies.
  2. Protein Expression in bacteria, baculovirus- insect cell and mammalian cell systems
  3. Providing rapid and robust solutions for purification of high quality protein using different liquid handling chromatography systems (1x Akta Avant, 2x AKTAxpress, 1x AktaFPLC)
  4. Measurement and analysis of protein-protein, protein-nucleic acids and drug-protein interactions. (Biacore T100, ITC, Nanolith NT115)
  5. Determination of the native molar mass, oligomeric state and protein-protein complex formation using the size-exclusion multi Angle light scattering (SEC-MALS) instrument
  6. CRISPR-Cas9 genome engineering technology
  7. Automation for screening assays (Tecan evo200 and Sciclone robotic platforms)

Research Projects

  • Genome editing reagents for CRISPR-Cas9 applications
  • Implementation of protein-protein and protein-RNA screening assays
  • In house enzymes production to facilitate implementation of novel genomic applications (in collaboration with the Genomics Unit)

Training and dissemination activities

The unit organized in October the 3rd five-day Courses@CRG on “CRISPR-Cas9 tool: From Gene to Function” for 20 international participants and with five invited speakers that are leaders in the field.

All members of the staff are frequently participating as speakers and instructors in master courses from the CRG and UPF.